Complete Genome Sequences of a Mycobacterium smegmatis Laboratory Strain (MC2 155) and Isoniazid-Resistant (4XR1/R2) Mutant Strains
نویسندگان
چکیده
We report the whole genome sequences of a Mycobacterium smegmatis laboratory wild-type strain (MC(2) 155) and mutants (4XR1, 4XR2) resistant to isoniazid. Compared to Mycobacterium smegmatis MC(2) 155 (NC_008596), a widely used strain in laboratory experiments, the MC(2) 155, 4XR1, and 4XR2 strains are 60, 128 and 93 bp longer, respectively.
منابع مشابه
Draft Genome Sequence of mc251, a Highly Hydrogen Peroxide-Resistant Mycobacterium smegmatis Mutant Strain
Here, we report the draft genome sequence of the Mycobacterium tuberculosis-like Mycobacterium smegmatis mutant strain, mc(2)51, compared to that of wild-type strain M. smegmatis mc(2)155. The draft genome sequence comprises 6,823,739 bp, revealing 6,569 coding DNA sequences (CDSs) and 50 RNA genes (4 rRNA genes and 46 tRNA genes).
متن کاملGene expression profiles of wild-type and isoniazid-resistant strains of Mycobacterium smegmatis
The global variations in the gene expression pattern of drug treated (½X) and laboratory evolved drug-resistant strains (2XR and 4XR) of Mycobacterium smegmatis were obtained and compared with the M. smegmatis mc(2) 155 (WT) strain. The genes exhibiting two-fold change and p-value <0.05 under the treated conditions have been considered as differentially expressed genes (DEGs). Overall, the numb...
متن کاملIdentification of the regulator gene responsible for the acetone-responsive expression of the binuclear iron monooxygenase gene cluster in mycobacteria.
The mimABCD gene cluster encodes the binuclear iron monooxygenase that oxidizes propane and phenol in Mycobacterium smegmatis strain MC2 155 and Mycobacterium goodii strain 12523. Interestingly, expression of the mimABCD gene cluster is induced by acetone. In this study, we investigated the regulator gene responsible for this acetone-responsive expression. In the genome sequence of M. smegmatis...
متن کاملMycobacterium smegmatis mc2 155 fbiC and MSMEG_2392 are involved in triphenylmethane dye decolorization and coenzyme F420 biosynthesis.
Mycobacteria can tolerate relatively high concentrations of triphenylmethane dyes such as malachite green and methyl violet. To identify mycobacterial genes involved in the decolorization of malachite green, a transposon mutant library of Mycobacterium smegmatis mc2 155 was screened for mutants unable to decolorize this dye. One of the genes identified was MSMEG_5126, an orthologue of Mycobacte...
متن کاملMycobacterium smegmatis is a suitable cell factory for the production of steroidic synthons
A number of pharmaceutical steroid synthons are currently produced through the microbial side-chain cleavage of natural sterols as an alternative to multi-step chemical synthesis. Industrially, these synthons have been usually produced through fermentative processes using environmental isolated microorganisms or their conventional mutants. Mycobacterium smegmatis mc2 155 is a model organism for...
متن کامل